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目的：探讨辐射损伤对肠粘膜免疫的影响。方法：小鼠分别受３Ｇｙ、８Ｇｙ、１２Ｇｙ全身照射后８ｈ至１５ｄ６个时相，分离全小肠上皮细胞内淋巴细胞（Ｉｎｔｒａｅｐｉｔｈｅｌｉａｌｌｙｍｐｈｏｃｙｔｅ，ＩＥＬ），用佛波酯、ＣｏｎＡ联合ｒｈＩＬ－２刺激，以３Ｈ－ＴｄＲ参入的计数值反映增殖活力。结果：①３种剂量组ＩＥＬ增殖活力下降明显，都表现出以伤后８ｈ下降，７２ｈ达低值，然后逐渐回升的过程；１２Ｇｙ组的抑制出现更早（８ｈ），程度更重；两种刺激剂的变化态势相同。②正常小鼠分离的ＩＥＬ经体外照射１Ｇｙ～１０Ｇｙ亦导致增殖活力下降；３Ｇｙ和８Ｇｙ同一剂量的整体和离体照射相比较，对增殖活力抑制程度无明显的差异。结论：３种剂量的照射都造成肠粘膜免疫功能的下降，导致肠道屏障功能的削弱
Objective: To investigate the effect of radiation injury on intestinal mucosal immunity. Methods: Intraepithelialmphocyte (IEL) was isolated from 8h to 15d after irradiation of whole body of 3Gy, 8Gy and 12Gy mice, stimulated by phorbol ester, ConA and rhIL-2, The count value of TdR incorporation reflects proliferative activity. Results: (1) The proliferation of IELs in three different dose groups decreased significantly at 8h after injury and reached a low value at 72h after injury, and then gradually increased. The inhibition of IEL in 12Gy group was more severe (8h) The same trend of agents change. ② The IEL isolated from normal mice also decreased the viability of IG exposed to 1Gy ~ 10Gy. The same dose of 3Gy and 8Gy showed no significant difference in the inhibitory effect on proliferative activity compared with that in vitro. Conclusion: All the three doses of irradiation cause the decrease of intestinal mucosal immune function, leading to impaired intestinal barrier function