用反相HPLC 法测定镇痛药中醋氨酚咖、啡因和异丙安替比林(Propyphemazone)的含量,方法精密、正确、线性梯度能有效分离三个成份,不受杂质干扰并可分离出醋氨酚中杂质4-氯基酚和4-乙酰替氯苯胺。本法亦可测定其他镇痛药中安替比林、水杨酰胺和羟苯乙酯(Ethylparaben)等。实验采用C_(18)反相层析柱A25cm×4.6mm 内径,用涂以十八硅烷 The contents of acetaminophen, caffeine and Propyphemazone in analgesics were determined by reverse-phase HPLC. The method was precise and accurate, and the linear gradient could effectively separate the three components without interference from impurities The impurities 4-chlorophenol and 4-acetyl-chloroaniline in acetaminophen were isolated. This method can also be measured in other analgesics Antipyrine, salicylamide and ethyl ester (Ethylparaben) and so on. Experiments using C_ (18) reversed-phase column A25cm × 4.6mm diameter, coated with octadecane