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目的原位检测肝细胞癌（ＨＣＣ）中ＣＤ４４ｖ６ｍＲＮＡ及ｎｍ２３Ｈ１ｍＲＮＡ的表达，了解ＣＤ４４ｖ６ｍＲＮＡ及ｎｍ２３Ｈ１ｍＲＮＡ的表达与ＨＣＣ侵袭转移的关系，了解ＨＣＣ中ＣＤ４４ｖ６ｍＲＮＡ表达与ｎｍ２３Ｈ１ｍＲＮＡ表达的相关性．方法采用ＰＣＲ法合成ＣＤ４４ｖ６ｃＤＮＡ探针，体外转录法合成ｎｍ２３Ｈ１ｃＲＮＡ探针，采用原位杂交法检测ＨＣＣ３３例ＣＤ４４ｖ６ｍＲＮＡ和ｎｍ２３Ｈ１ｍＲＮＡ的表达．结果侵袭转移倾向高危组的１０例ＨＣＣ标本中，ＣＤ４４ｖ６ｍＲＮＡ和ｎｍ２３Ｈ１ｍＲＮＡ的阳性率分别为８００％（８／１０）和４００％（４／１０）；侵袭转移倾向低危组的２３例ＨＣＣ标本中，ＣＤ４４ｖ６ｍＲＮＡ和ｎｍ２３Ｈ１ｍＲＮＡ的阳性率分别为２１７％（５／２３）和９１３％（２１／２３）．ＣＤ４４ｖ６ｍＲＮＡ的表达与ＨＣＣ的侵袭转移倾向具有正相关性（Ｐ＜００１），ｎｍ２３Ｈ１ｍＲＮＡ的表达与ＨＣＣ的侵袭转移倾向具有负相关性（Ｐ＜００１），ＣＤ４４ｖ６ｍＲＮＡ及ｎｍ２３Ｈ１ｍＲＮＡ的表达具有负相关性（Ｐ＜００１）．结论检测ＣＤ４４ｖ６ｍＲＮＡ及ｎｍ２３Ｈ１ｍＲＮＡ的表达可能成为ＨＣＣ转移预后?
Objective To detect the expression of CD44v6 mRNA and nm23H1 mRNA in hepatocellular carcinoma (HCC) in situ, and to understand the relationship between the expression of CD44v6 mRNA and nm23H1 mRNA and the invasion and metastasis of HCC, and to understand the correlation between the expression of CD44v6 mRNA and the expression of nm23H1 mRNA in HCC. Methods The CD44v6 cDNA probe was synthesized by PCR and the nm23H1cRNA probe was synthesized by in vitro transcription method. The expression of CD44v6 mRNA and nm23H1 mRNA in 33 cases of HCC were detected by in situ hybridization. Results The positive rates of CD44v6 mRNA and nm23H1 mRNA in 10 HCC specimens with high risk of invasion and metastasis were 800% (8/10) and 400% (4/10), respectively; In 23 specimens of HCC, the positive rates of CD44v6 mRNA and nm23-H1 mRNA were 217% (5/23) and 913% (21/23), respectively. There was a positive correlation between the expression of CD44v6 mRNA and the invasion and metastasis tendency of HCC (P<001). The expression of nm23H1 mRNA was negatively correlated with the invasion and metastasis tendency of HCC (P<001), and the expression of CD44v6 mRNA and nm23H1 mRNA. The expression has a negative correlation (P<001). Conclusion The detection of CD44v6 mRNA and nm23-H1 mRNA may be the prognosis of HCC metastasis.