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目的 建立同时检测肺炎链球菌(Streptococcus pneumoniae,SP)、大肠杆菌(Escherichia coli,EC)、流感嗜血杆菌B型(Haemophilus influenzae type B,HIB)、脑膜炎奈瑟球菌(Neisseria meningitidis,NM)、无乳链球菌(Streptococcus agalactiae,GBS)、单核细胞增生李斯特菌(Listeria monocytogenes,LM)6种常见儿童化脓性脑膜炎病原菌的PCR阵列方法,为快速检测临床常见的化脓性脑膜炎病原菌提供新技术.方法 用6种病原菌核酸评价PCR阵列方法的扩增效率,最低检出限(LOD)及各病原引物探针之间是否存在交叉反应;利用模拟脑脊液样本评价PCR阵列方法的灵敏度与特异性,并收集临床脑脊液样本考察PCR阵列方法与临床脑脊液培养金标准方法的一致性.结果 PCR阵列中各病原菌的引物探针特异性良好,无交叉反应,LOD可达10 cfu/ml.在模拟样本的评价中,PCR阵列方法灵敏度与特异性均可达到95％,且与临床金标准方法有较好的一致性.结论 PCR阵列方法可实现2.5h内对6种常见儿童化脓性脑膜炎病原菌的同时检测,且灵敏度和特异性高,可为临床儿童化脓性脑膜炎病原学诊断提供参考.“,”Objective To develop a PCR-array method for detecting common purulent meningitis pathogens including Streptococcus pneumoniae,Escherichia coli,Haemophilus influenzae type B,Neisseria meningitidis,S.agalactiae and Listeria monocytogenes in children.Methods The amplification efficiency,limit of detection (LOD) and cross-reactivity were validated with individually real-time PCR using genomic DNA of the six pathogenic bacteria.The sensitivity and specificity of the PCR-array method were evaluated using artificial cerebrospinal fluid(CSF),and the consistency between the PCR-array method and the golden method of CSF culture was evaluated using clinical samples.Results The primers and probes of the pathogens in PCR-array had high specificity,and there was no cross reaction between them.The LOD of the PCR-array method was 10 cfu/ml and very sensitive.The sensitivity and specificity of the PCR-array method could reach 95％ in the evaluation of artificial CSF,and had a good consistency with the clinical gold standard method.Conclusion The PCR-array method with high sensitivity and specificity can simultaneously detect six common pathogens in children with purulent meningitis at 2.5 h,which could provide reference for the diagnosis of purulent meningitis.