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提出了利用寡核苷酸探针快速筛选重组克隆的方法,在数小时内即可完成杂交和自显影的过程。以克隆羊β-乳球蛋白基因(BLG)第一内含子为例进行了说明。
A rapid method of screening recombinant clones using oligonucleotide probes is proposed to accomplish hybridization and autoradiography in a matter of hours. An example of the first intron of the cloned sheep β-lactoglobulin gene (BLG) was described.