MRC-9细胞是1974年得自一15周龄女性胎儿的肺组织,经胰酶消化、连续传代培育的成纤维细胞。该胎儿的母亲14岁,本人及家族无异常病史。胎儿发育正常,其母因未婚怀孕行人工流产。培养细胞的营养液为含8％牛血清及15mM HEPES缓冲液的 MRC-9 cells were lung tissue obtained from a 15-week-old female fetus in 1974 and were serially passaged to culture fibroblasts by trypsinization. The fetus’ mother is 14 years old and has no abnormal history of himself and his family. Fetal development is normal, her maternal unmarried pregnancy abortion. The nutrient solution for cell culture is 8% bovine serum and 15 mM HEPES buffer