论文部分内容阅读
目的研究人肝再生增强因子(augmenter of liver regenerationALR)205基因对大鼠急性肝损伤的治疗作用。方法将200μg/kg重组质粒PCDNA3.1(+)-ALR205尾静脉注射给CCL4致急性肝损伤大鼠,检测血清AST、ALT水平,肝组织病理学变化、肝组织增殖细胞核抗原(Proliferating cell nuclear antigenPCNA)并对照ALR125基因治疗来观察ALR205基因对急性肝损伤的疗效。RT-PCR检测肝组织ALR205 mRNA表达情况。结果重组质粒用于急性肝损伤大鼠后,血清酶学检查结果表明外周血AST、ALT水平显著降低,肝组织病理损害情况大幅度减轻,治疗组肝PCNA指数均明显高于其它组且ALR205比ALR125在促肝细胞增殖方面有明显差异。结论ALR205基因体内表达产物通过促进肝细胞增殖,降低血清AST、ALT水平发挥抗急性肝损伤作用。
Objective To investigate the therapeutic effect of augmenter of liver regenerationALR 205 gene on acute liver injury in rats. Methods 200 μg / kg PCDNA3.1 (+) - ALR205 was injected into the tail vein of CCL4-induced acute liver injury rats to detect the levels of AST and ALT, the pathological changes of hepatic tissue and the expression of Proliferating cell nuclear antigen PCNA ) And control ALR125 gene therapy to observe the ALR205 gene effect on acute liver injury. The expression of ALR205 mRNA in liver tissue was detected by RT-PCR. Results The recombinant plasmids were used in acute liver injury rats. The result of serum enzyme test showed that the level of AST and ALT in peripheral blood were significantly reduced, and the pathological damage in liver tissues was significantly reduced. The PCNA index in treatment group was significantly higher than that in other groups and ALR205 ALR125 in promoting hepatocyte proliferation have significant differences. Conclusion The expression of ALR205 gene in vivo plays an anti-acute liver injury role by promoting hepatocyte proliferation, decreasing serum AST and ALT levels.