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目的通过对防风抽薹前后生长素(IAA)、赤霉素(GA)和脱落酸(ABA)的测定,明确了防风抽薹前后IAA、GA和ABA的量变化,为进一步研究防风早期抽薹机制提供理论依据。方法利用HPLC法测定抽薹前后IAA、GA和ABA量,色谱条件为C18 ODS反相柱,柱温35℃,检测波长254 nm,体积流量0.7 mL/min,流动相为甲醇-乙腈-0.7%乙酸(40∶15∶45)。结果防风在抽薹期根、茎、叶中IAA、GA的量变化趋势基本相同,抽薹后IAA、GA的量则急剧下降,且抽薹前防风IAA、GA的量明显高于抽薹后。ABA在防风抽薹期根、茎、叶中的量变化趋势基本相同,但与IAA、GA差异较大。抽薹前取样ABA的量虽略有起伏,抽薹后ABA的量急剧上升,且明显高于抽薹前。结论防风早期抽薹现象与防风内源激素水平有一定的关系,可以初步推断防风内源激素量的变化导致了防风的早期抽薹。
OBJECTIVE: To determine the changes of IAA, GA and ABA before and after windbreak bolting by determining the contents of IAA, GA and ABA before and after bolting, and to provide theoretical basis for further study on bolting mechanism in windbreak. in accordance with. Methods The contents of IAA, GA and ABA before and after bolting were determined by HPLC. The chromatographic conditions were C18 ODS reversed-phase column. The column temperature was 35 ℃, the detection wavelength was 254 nm, the volume flow rate was 0.7 mL / min and the mobile phase was methanol-acetonitrile- (40:15:45). Results The trend of IAA and GA in roots, stems and leaves of windbreak was basically the same at the bolting stage. The amount of IAA and GA dropped sharply after bolting, and the IAA and GA contents of windbreak before bolting were obviously higher than those after bolting. The trend of ABA content in roots, stems and leaves at windbreak bolting stage was basically the same, but different from that of IAA and GA. Although the amount of ABA sampled before bolting was slightly fluctuated, the amount of ABA after bolting increased sharply and was significantly higher than that before bolting. Conclusion It is concluded that bolting in the early stage of windbreak has a certain relationship with the level of endogenous hormones in windbreak. It can be preliminarily concluded that the change of endogenous hormones in windbreak leads to the early bolting of windbreak.