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研究转化生长因子β1(TGF-β1)+869T/C基因多态性与甲状腺疾病的相关性。采用序列特异性引物聚合酶链反应(PCR-sequence specific primers,PCR-SSP)方法,检测136例结节性甲状腺肿伴甲亢患者,132例毒性甲状腺腺瘤患者,185例Graves病患者,202例桥本甲状腺炎患者,240例正常对照组的TGF-β1+869T/C基因多态性。结果表明:1.TGF-β1+869T/C的CC基因型及C等位基因频率分布在结节性甲状腺肿伴甲亢、毒性甲状腺腺瘤显著高于正常对照,组间差异有统计学意义(P<0.05);2.结节性甲状腺肿伴甲亢与毒性甲状腺腺瘤合称甲亢有结节组,其CC基因型及C等位基因频率分布显著高于单纯Graves病组,二者比较差异有统计学意义(P<0.05);3.有结节的HT组的TGF-β1+869T/C的CC基因型及C等位基因频率分布显著高于无结节的HT组,组间比较差异有统计学意义(P<0.05)。提示:TGF-β1基因+869T/C可能与结甲伴甲亢、毒性腺瘤的发病有关,而与GD、HT发病无关;“C”等位基因可能是包头地区汉族人群中结节性甲状腺肿伴甲亢、毒性甲状腺腺瘤的易感因素之一。CC基因型与C等位基因可能与甲状腺疾病中的结节存在相关性。
To investigate the relationship between TGF-β1 + 869T / C polymorphism and thyroid diseases. A total of 136 patients with nodular goiter with hyperthyroidism, 132 patients with toxic thyroid adenoma, 185 patients with Graves ’disease and 202 patients with Graves’ disease were detected by PCR-S specific PCR (PCR-S specific PCR) Hashimoto’s thyroiditis patients, 240 normal control group TGF-β1 + 869T / C gene polymorphisms. The results showed that: 1. The frequency of CC genotype and allele C of TGF-β1 + 869T / C was significantly higher in nodular goiter with hyperthyroidism and toxic thyroid adenoma than in normal controls (P <0.05) P <0.05); 2. Nodular goiter with hyperthyroidism and toxic thyroid adenoma together called hyperthyroidism nodules, the CC genotype and C allele frequency distribution was significantly higher than the simple Graves disease group, the difference between the two (P <0.05) .3. The frequency of CC genotype and C allele of TGF-β1 + 869T / C in nodular HT group was significantly higher than that in non-nodular HT group The difference was statistically significant (P <0.05). It is suggested that TGF-β1 gene + 869T / C may be related to the pathogenesis of hyperthyroidism and adenoma with the exception of GD and HT. The “C” allele may be the nodular Goiter with hyperthyroidism, one of the susceptible factors of toxic thyroid adenoma. CC genotype and C allele may be related to nodules in thyroid disease.