目的研究人脐带间充质干细胞(human umbilical cord mesenchymal stem cells,h UCMSCs)对小鼠全层皮肤切除创面愈合及血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)表达的影响。方法采用组织块贴壁法分离培养h UC-MSCs,流式细胞术检测细胞免疫表型,并做向成骨、成脂细胞方向分化。48只雄性BALB/c小鼠采用完全随机分组方法分为h UC-MSCs组和PBS组,每组24只,在其背部形成一全层皮肤缺损创面模型,分别于创周注射h UC-MSCs悬液和PBS液。于3、7、14 d观察创面愈合情况,HE染色观察病理学改变,免疫组织化学及Western blot检测VEGF蛋白的表达。结果流式细胞术检测显示所培养的细胞为h UC-MSCs,体外诱导其能向成骨、成脂细胞方向分化。h UC-MSCs组创面愈合率在各时间点均较PBS组显著增高(P<0.05)。HE染色显示h UCMSCs组创面愈合质量优于PBS组。免疫组化显示h UC-MSCs组VEGF在3 d时表达最多,7、14 d逐渐减少,但均较PBS组增高。Western blot结果显示h UC-MSCs组3、7、14 d创面VEGF蛋白表达显著高于PBS组(P<0.05)。结论 h UC-MSCs可增强皮肤创面VEGF的表达,促进皮肤创面的修复。 Objective To study the effects of human umbilical cord mesenchymal stem cells (hUCMSCs) on the healing of wounds and the expression of vascular endothelial growth factor (VEGF) in full-thickness skin of mice. Methods The hUC-MSCs were isolated and cultured by tissue block method. The cellular phenotypes were detected by flow cytometry and differentiated into osteoblasts and adipocytes. 48 male BALB / c mice were randomly divided into h UC-MSCs group and PBS group, 24 rats in each group, forming a full-thickness skin defect wound model on their backs. Suspension and PBS solution. The wound healing was observed on days 3, 7 and 14, pathological changes were observed by HE staining, and the expression of VEGF protein was detected by immunohistochemistry and Western blot. Results Flow cytometry showed that hUC-MSCs cultured in vitro were induced to differentiate into osteoblasts and adipocytes in vitro. h UC-MSCs wound healing rate at each time point was significantly higher than the PBS group (P <0.05). HE staining showed that the wound healing of h UCMSCs group was better than PBS group. Immunohistochemistry showed that the expression of VEGF in h UC-MSCs group was the highest at 3 days and gradually decreased at 7 and 14 days, all of which were higher than those in PBS group. Western blot results showed that VEGF protein expression in hUC-MSCs group was significantly higher than that in PBS group on the 3rd, 7th and 14th day (P <0.05). Conclusion h UC-MSCs can enhance the expression of VEGF in skin wounds and promote the repair of skin wounds.