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以“青芋1号”和“神芋”菊芋品种为试材,通过自然状态的低温锻炼,测定了2个菊芋品种块茎中总糖、还原糖、可溶性糖及淀粉含量的变化;并利用扫描透射电镜观察了低温锻炼下的块茎细胞超微结构。结果表明:抗寒性品种“青芋1号”随温度降低,总糖含量升高,还原糖含量升高,可溶性糖含量增加,淀粉含量降低;反之,非抗寒性品种“神芋”总糖含量降低,还原糖升高幅度小,可溶性糖含量降低,淀粉含量降低幅度大。细胞超微结构发现,抗寒性品种“青芋1号”在-20℃时才表现出液泡破碎,线粒体数量增多,变形及解体;非抗寒性品种“神芋”在5℃时就出现类似现象,这与植物在遭受寒冷环境条件细胞适应性变化相一致。
The changes of total sugar, reducing sugar, soluble sugar and starch content in tubers of two Jerusalem artichoke cultivars were determined by low temperature exercise of “Yajian No.1” and “Calla” cultivars The ultrastructure of tuber cells under cold stress was observed by scanning electron microscopy. The results showed that the cold resistance cultivar “Goji No.1” decreased with temperature, the total sugar content increased, the content of reducing sugar increased, the content of soluble sugar increased, and the starch content decreased. On the contrary, Taro “total sugar content decreased, reducing sugar increased small, soluble sugar content decreased, starch content decreased by a large extent. The ultrastructure of the cells showed that cold resistance variety ”Hot potato No.1“ showed vacuolar fragmentation, increased number of mitochondria, deformation and disintegration at -20 ℃. In non-resistant variety ”Calla" at 5 Similar phenomenon occurs when the temperature is higher than ℃, which is consistent with the change of plant adaptability to cold conditions.