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目的研究环境暴露水平的六溴环十二烷(HBCD)对发育期大鼠的胆碱能神经系统的神经毒性作用。方法将35只3日龄清洁级Sprague-Dawley(SD)雌性大鼠随机分为5组,分别为对照(花生油)组和10、50、100、300μg/kg HBCD染毒组,每组7只。采用灌胃方式进行染毒,染毒容量为1 ml/kg,连续染毒42 d。测定脑组织中乙酰胆碱(Ach)的含量及乙酰胆碱酯酶(AchE)和乙酰胆碱转移酶(ChAT)的活力。结果与对照组比较,100、300μg/kg HBCD染毒组雌性大鼠脑组织中Ach的含量降低,300μg/kg HBCD染毒组脑组织中AchE活力和10μg/kg HBCD染毒组脑组织中ChAT活力升高,差异均有统计学意义(P<0.05)。结论 HBCD染毒可对脑发育期大鼠的胆碱能系统产生损伤作用,进而对神经发育产生影响。
Objective To investigate the neurotoxic effects of HBCD on the cholinergic nervous system in developing rats. Methods Thirty-five 3-day-old female Sprague-Dawley rats were randomly divided into 5 groups: control (peanut oil) group and 10, 50, 100, 300μg / kg HBCD exposure groups . The method of gavage was used to inoculate and the volume of infection was 1 ml / kg for 42 days. The content of acetylcholine (Ach) and the activity of acetylcholinesterase (AchE) and acetylcholinesterase (ChAT) in brain were measured. Results Compared with the control group, the content of Ach in the brain of 100,300μg / kg HBCD-treated female rats decreased. The AchE activity in brain tissue of 300μg / kg HBCD-treated rats and ChAT Vitality increased, the difference was statistically significant (P <0.05). Conclusion HBCD can injure the cholinergic system in brain development rats and then affect the neurodevelopment.