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目的研究枫蓼提取物中主要化学成分的抗炎解痉、止泻作用,探索其治疗急性胃肠炎的药效物质。方法采用小鼠耳肿胀和大鼠足肿胀炎症模型,分别测量耳和足肿胀度,测定大鼠炎足组织中TNF-α、IL~(-1)β和PGE2含量,观察槲皮素和芦丁的抗炎作用。采用小肠推进和番泻叶致小鼠腹泻实验,以小肠推进率、稀便率、腹泻指数为指标,研究槲皮素和芦丁的解痉、抗腹泻作用。结果槲皮素和芦丁能减轻小鼠耳肿胀和大鼠足肿胀,不同程度降低炎足组织中TNF-α、IL~(-1)β和PGE2含量,抑制小肠推进;减少腹泻次数、降低腹泻指数。结论槲皮素、芦丁具有明显抗炎、解痉和抗腹泻作用,是枫蓼提取物治疗急性胃肠炎的主要有效成分之一。
Objective To study the anti-inflammatory, antispasmodic and anti-diarrhea effects of the main chemical constituents from the leaves of Polygonum amygdalifolia and to explore its therapeutic agents for the treatment of acute gastroenteritis. Methods Ear swelling and rat foot swelling model were used to measure ear and foot swelling. The levels of TNF-α, IL-1 β and PGE2 in rat inflammatory foot tissues were measured, and the contents of quercetin, Ding anti-inflammatory effect. Using small intestine propulsion and diarrhea induced by senna in mice, we studied the antispasmodic and anti-diarrhea effects of quercetin and rutin by using small intestine propulsion rate, loose stool rate and diarrhea index as indexes. Results Quercetin and rutin could relieve ear edema and rat foot swelling in mice, and reduce the content of TNF-α, IL-1β and PGE2 in the inflammation foot tissues to a certain extent, and inhibit the progression of small intestine; reduce the frequency of diarrhea and decrease Diarrhea index. Conclusions Quercetin and rutin have obvious anti-inflammatory, antispasmodic and anti-diarrhea effects, and are one of the main active ingredients in the treatment of acute gastroenteritis by Fengliao extract.