克山病是一种原因未明的心肌病,其基本病理过程是心肌的多发性灶状坏死和修复。心肌细胞坏死的方式,可分为凝固性坏死和溶解性坏死两种类型,这在光学显微镜下已有大量的观察和描述。近些年来对克山病心肌病变已有少数电镜观察报告,对各种细胞器的改变作了一些描述,但是从超微结构的水平对基本病变的研究还是很不够的。为了加深对克山病病变本质的了解,我们设计了一种“细胞定位”技术,并改进了环氧树脂包埋组织 Keshan disease is an unknown etiology of cardiomyopathy, the basic pathological process is multiple focal necrosis and repair of myocardium. Myocardial cell necrosis can be divided into two types of coagulation necrosis and lytic necrosis, which under the optical microscope has been a lot of observation and description. In recent years, Keshan disease has been found in a small number of electron microscopic observation of myocardial lesions, changes in a variety of organelles made some description, but the basic level of the ultrastructure of the study is still not enough. In order to deepen the understanding of the nature of Keshan disease, we designed a “cell localization” technique and improved the epoxy-embedded tissue