论文部分内容阅读
大多数植物病毒都含有单链RNA基因组,在感染细胞内以双链形式复制。在感染病毒的植物体内经常可以检测到这种全长和某些非全长双链RNA(dsRNA)。通常健康植物检测不到。因此,植物提取液中dsRNA的存在是病毒感染的极好证据。以往利用酚从组织中提取核酸,用CF-11纤维素柱层析法纯化dsRNA,再用聚丙烯酰胺凝胶电泳法(PAGE)分离不同大小的dsRNA。随着血清学技术的发展,应用合成多核苷酸(如多聚肌苷-胞苷酸(简称In-Cn),或多聚腺苷-尿苷酸(简称An-Un)的单克隆或多克隆抗体可以快
Most plant viruses contain a single-stranded RNA genome that replicates in double-stranded form in infected cells. This full length and some non-full length double stranded RNA (dsRNA) can often be detected in virus-infected plants. Usually healthy plants can not be detected. Therefore, the presence of dsRNA in plant extracts is excellent evidence of viral infection. In the past, nucleic acids were extracted from tissues using phenol, dsRNAs were purified by CF-11 cellulose column chromatography, and dsRNAs of different sizes were separated by polyacrylamide gel electrophoresis (PAGE). With the development of serological techniques, the use of synthetic polynucleotides (such as poly-inosinic acid (abbreviated as In-Cn), or poly-adenylate (abbreviated An-Un) Clonal antibodies can be fast