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目的:建立同时测定荆芥药材中薄荷酮与胡薄荷酮的含量的方法。方法:采用超声溶剂提取法对样品进行提取后,用HP-5气相色谱柱(以5%苯基甲基硅氧烷为固定液,30m×0.32mm×0.25μm)在气相色谱仪(配FID检测器)中进行测定。结果:薄荷酮在0.002-5.0g/L(r=0.9999)范围,胡薄荷酮在0.002-5.0g/L(r=0.9996)范围,线性良好。薄荷酮与胡薄荷酮的回收率分别为96.3%-103.9%和95.7%-102.5%。结论:所建立的方法准确、简便,可同时测定荆芥药材中的薄荷酮与胡薄荷酮的含量。
Objective: To establish a method for the simultaneous determination of menthone and pulegone in Nepeta medicinal herbs. Methods: The samples were extracted by ultrasonic solvent extraction and analyzed by gas chromatography (FID) with HP-5 gas chromatography column (5% phenylmethylsiloxane as fixative solution, 30m × 0.32mm × 0.25μm) Detector). Results: The concentrations of menthone in the range of 0.002-5.0 g / L (r = 0.9999) and the concentration of pulegone in the range of 0.002-5.0 g / L (r = 0.9996) were good. The recoveries of menthone and pulegone were 96.3% -103.9% and 95.7% -102.5%, respectively. Conclusion: The established method is accurate and simple. Simultaneous determination of menthone and pentmentone in Nepeta medicinal herbs can be carried out.