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目的了解宁波市江东区感染性腹泻病原构成情况及流行季节性特点。方法对全区所有腹泻门诊肛拭子或粪便样品采用直接划线分离、增菌培养,生化鉴定、血清分型等方法,检测霍乱弧菌、副溶血性弧菌、沙门菌、志贺菌和出血性大肠杆菌O157:H7等肠道致病菌,对其中的61份样品进行病毒核酸检测;分离到的副溶血性弧菌进行血清分型和药物敏感试验。结果 335份腹泻样品病原菌检出率为11.9%(40/335),其中副溶血性弧菌占85.0%(34/40),志贺菌占7.50%(3/40)。副溶血性弧菌优势血清型为O3群,占73.5%(25/34),未检出霍乱弧菌和出血性大肠杆菌O157:H7。61份检测轮状和诺如病毒核酸样品,病毒核酸阳性27例,检出率44.3%(27/61),其中诺如病毒Ⅱ型阳性63.0%(17/27),轮状病毒核酸A组型阳占33.3%(9/27)。结论江东区夏秋季散发感染性腹泻的主要致病菌为副溶血性弧菌;在冬秋春季腹泻样品中诺如病毒、轮状病毒分离率高。
Objective To understand the pathogenic constitution and seasonal characteristics of infectious diarrhea in Jiangdong District of Ningbo City. Methods Vibrio cholerae, Vibrio parahaemolyticus, Salmonella spp., Shigella spp. And Vibrio parahaemolyticus were detected by direct scribing, enrichment culture, biochemical identification and serotyping on all the anus swabs or stool samples from outpatients in the district. Hemorrhagic Escherichia coli O157: H7 and other enteric pathogens, of which 61 samples for viral nucleic acid detection; isolated Vibrio parahaemolyticus serum typing and drug sensitivity tests. Results The detection rate of pathogenic bacteria in 335 diarrhea samples was 11.9% (40/335), of which 85.0% (34/40) were Vibrio parahaemolyticus and 7.50% (3/40) were Shigella. Vibrio parahaemolyticus dominant serotype O3 group, accounting for 73.5% (25/34), did not detect Vibrio cholerae and hemorrhagic Escherichia coli O157: H7.61 detection of rotavirus and Norovirus nucleic acid samples, viral nucleic acid The positive rate was 27.3% (27/61). The positive rate of Norovirus type Ⅱ was 63.0% (17/27) and that of rotavirus type A was 33.3% (9/27). Conclusions The main pathogen of infectious diarrhea in Jiangdong district is Vibrio parahaemolyticus in summer and autumn. Noroviruses and rotavirus in diarrhea samples in winter and autumn are highly isolated.