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目的 证实大鼠腹膜表面液体层的存在并探讨其可能组成成分。 方法 取正常大鼠腹膜组织 ,分别以不同固定液固定 :(1) 2 .5 %戊二醛和 2 %多聚甲醛 (对照组 ) ;(2 ) 2 .5 %戊二醛和 0 .5 %氯化十六烷基吡啶 (GAG组 ) ;(3) 2 % Os O4(PH 1组 ) ;(4 ) 4 % Os O4(PH2组 ) ;(5 ) 3%鞣酸 (鞣酸组 )。 结果 对照组中可见腹膜间皮细胞表面的微绒毛 ,未见表面液体层。GAG组腹膜表面可见一层不连续的无定形结构。此层结构在 PH1和 PH 2组中保存较好 ,厚度达到 10μm。鞣酸组表面液体层及间皮细胞中可见许多类板层小体。 结论 研究证实 :正常大鼠腹膜表面覆盖着一层膜样结构 ,它至少由磷脂和葡糖胺聚糖组成。该层在腹膜物质转运中可能有重要的屏障作用。
Objective To confirm the existence of liquid layer on rat peritoneal surface and to explore its possible components. Methods Normal rat peritoneal tissue was taken and fixed with different fixative solutions: (1) 2.5% glutaraldehyde and 2% paraformaldehyde (control group); (2) 2.5% glutaraldehyde and 0.5 (GAG group); (3) 2% Os O4 (PH 1 group); (4) 4% Os O4 (PH2 group); . Results The control group showed microvilli on the surface of peritoneal mesothelial cells, no surface liquid layer. GAG group peritoneal surface can be seen a layer of non-continuous amorphous structure. This layer structure is well preserved in the PH1 and PH2 groups with a thickness of 10 μm. Tannic acid group surface liquid layer and mesothelial cells can be seen in many types of lamellar body. Conclusions The study demonstrated that the normal rat peritoneal surface is covered with a membrane-like structure consisting at least of phospholipids and glycosaminoglycans. This layer may have an important barrier function in peritoneal material transport.