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目的检测HPV L1和16/18DNA在喉癌组织中的表达,探讨HPV在喉癌发生中的作用。方法用原位杂交(ISH)检测123例喉癌组织及123例“正常人”的喉粘膜上皮组织中HPV-16/18 mRNA。结果在123例喉癌标本中,ISH检出HPV-16E6的阳性率为46.34%,HPV-18E6的阳性率为35.77%。123例“正常人”的喉粘膜ISH结果表明:低危型HPV的阳性率与喉癌相近;高危型HPV的阳性率虽远远低于喉癌,但随着组织病变程度加重阳性率逐渐升高。对上述结果用SPSS 10.0软件进行case-controlχ2分析,HPV-16感染会增加喉上皮病变的风险(OR=14.58),感染HPV-18时,喉上皮病变的风险(OR=10.77)。结论HPV-16感染是重庆地区喉癌的重要发病因素;HPV-16感染后E6片段的保留并持续表达与喉组织的癌变进程密切相关;HPV-16感染会增强喉上皮病变的风险,而HPV-18有协同作用。
Objective To detect the expression of HPV L1 and 16/18 DNA in laryngeal carcinoma and to explore the role of HPV in laryngeal carcinogenesis. Methods HPV-16/18 mRNA was detected by in situ hybridization (ISH) in 123 cases of laryngeal carcinoma and 123 cases of normal human laryngeal epithelium. Results In 123 specimens of laryngeal cancer, the positive rate of HPV16E6 detected by ISH was 46.34% and the positive rate of HPV-18E6 was 35.77%. 123 cases of “normal” the results of laryngeal mucosal ISH: low-risk HPV positive rate and laryngeal cancer is similar; high-risk HPV positive rate is much lower than laryngeal cancer, but as the degree of tissue lesions increased the positive rate Gradually increased. Case-control 2 analysis of the above results with SPSS 10.0 software showed that HPV-16 infection increased the risk of laryngeal epithelial lesions (OR = 14.58) and the risk of laryngeal epithelial lesions (OR = 10.77) when infected with HPV-18. Conclusion HPV-16 infection is an important risk factor for laryngeal cancer in Chongqing. The retention and persistence of E6 fragments after HPV-16 infection is closely related to the carcinogenesis of laryngeal tissue. HPV-16 infection may increase the risk of laryngeal epithelial lesions. HPV- -18 have synergy.