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应用３０ Ｌ生物反应器和微载体悬浮 培养技术，通过电脑全自动控制，连续灌注培养分泌ＨＢｓＡｇ 的重组ＣＨＯ－Ｃ２８细胞。试验了培养方式、连续灌流速度，反应器转速和细胞对葡萄糖消耗等工艺条件。观察培养６０ 天，细胞的生长形态、ＨＢｓＡｇ 分泌动态和染色体数。研究结果表明，连续培养６０ 天，细胞密度可达７．０×１０６ｃｅｌｌｓ／ｍ ｌ，平均维持在（５．０～６．０）×１０６ｃｅｌｌｓ／ｍ ｌ，收液的ＲＰＨＡ 滴度可达１∶５１２，ＨＢｓＡｇ 每天平均产量为３０ ｍ ｇ。
Using 30 L bioreactor and microcarrier suspension culture technology, the recombinant CHO-C28 cells secreting HBsAg were continuously perfused by computerized automatic control. The culture conditions, continuous perfusion rate, reactor speed and cell-to-glucose consumption were tested. Observed for 60 days, the cell growth morphology, HBsAg secretion dynamics and chromosome number. The results showed that the cell density was up to 7.0 × 106cells / m l after continuous culture for 60 days, the average maintained at (5.0 ~ 6.0) × 106cells / ml. 512, the average daily output of HBsAg is 30 m g.