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目的:探讨可溶性PD-1(soluble PD-1,s PD-1)和调节性T细胞(Regulatory T lymphocyte,Treg)在肺炎支原体(mycoplasma pneumoniae,MP)感染与哮喘模型小鼠关系中的作用。方法:20只BALB/c小鼠随机分为4组(n=5),其中2组建立鸡卵白蛋白(ovalbumin,OVA)致敏哮喘模型,将MP菌液滴入其中1组OVA小鼠和1组对照小鼠,形成OVA致敏并MP感染组,OVA致敏组,MP感染组及正常对照组。采用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)方法分别检测各组小鼠血清、肺泡灌洗液(Bronchoalveolar Lavage Fluid,BALF)中s PD-1的浓度;采用流式细胞术检测脾脏组织中Treg细胞的比例。结果:1与正常对照组相比,OVA致敏并感染MP组、单纯OVA致敏组和单纯MP感染组小鼠血清s PD-1的浓度均升高,差异有统计学意义(P<0.05),BALF中的s PD-1的浓度亦增高,但无统计学差异;2与正常对照组相比,OVA致敏并感染MP组、单纯OVA致敏组小鼠的脾脏Treg细胞占淋巴细胞的比例下降,而单纯MP感染组Treg细胞的比例升高,差异具有统计学意义(P<0.05)。结论:s PD-1和Treg细胞可能参与了哮喘小鼠感染MP的发病。
Objective: To investigate the role of soluble PD-1 (s PD-1) and regulatory T lymphocyte (Treg) in the relationship between mycoplasma pneumoniae (MP) infection and asthma model mice. Methods: Twenty BALB / c mice were randomly divided into four groups (n = 5). Two groups were immunized with ovalbumin (OVA) -induced asthma model. One group of OVA mice One group of control mice formed OVA sensitized and MP infected group, OVA sensitized group, MP infected group and normal control group. The concentrations of s PD-1 in serum and bronchoalveolar lavage fluid (BALF) of mice were detected by enzyme linked immunosorbent assay (ELISA). The spleen tissue was detected by flow cytometry Treg cells in the ratio. Compared with normal control group, the concentration of s PD-1 in OVA-sensitized and infected MP group, OVA-sensitized group and MP-only group were significantly higher than that in normal control group (P <0.05 ), The concentration of s PD-1 in BALF also increased, but there was no statistical difference.2 Compared with the normal control group, OVA sensitized and infected the MP group, and the spleen Treg cells in the OVA sensitized group accounted for the lymphocytes (P <0.05). However, the proportion of Treg cells in pure MP infection group was significantly higher than that in control group (P <0.05). Conclusion: s PD-1 and Treg cells may be involved in the pathogenesis of MP infection in asthmatic mice.