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新的半合成力复霉素类抗生素异丁基哌嗪力复霉素的氯仿溶液呈黄绿色,在450mμ处有特征的光吸收峰,本文利用此原理用氯仿提取尿中的异丁基哌嗪力复霉素并比色测定其含量,观察了药物在体内的吸收、代谢和排泄情况。 被测试的病员9人,清晨5时排空尿液后服药(四川抗菌素工业研究所生产)200mg,分另,J收集5～7、7～9、11～13、13～15、15～17、17～20、20～次晨5时的尿液,并记录体积。取尿液3ml于离心管中,加入3ml氯仿,加盖后剧烈振荡100次,离心后用细滴管小心吸取下层氯仿提取液,在450mμ处以未服药健康人尿为对照空白,测定光吸收,再从空白尿中加入已知浓度的异丁基哌
The new semi-synthetic force of the compound antibiotics isobutylpiperazine erythromycin chloroform solution was yellow-green, at 450mμ at a characteristic light absorption peak, the paper uses this principle with chloroform extraction of urine isobutyl piperazine Cucurbitacin and colorimetric determination of its content, the observed drug absorption in the body, metabolism and excretion. The patient was tested 9, emptying the urine after 5:00 morning medication (Sichuan Institute of antibiotic production) 200mg, divided into another, J collected 5 ~ 7,7 ~ 9,11 ~ 13,13 ~ 15,15 ~ 17 , 17 ~ 20,20 ~ 5 o’clock morning urine, and record the volume. Take urine 3ml in centrifuge tube, add 3ml of chloroform, stamped with 100 times violent capillaries after centrifugation, carefully draw the lower chloroform extract at 450mμ to take unhealthy healthy urine as a control blank, measured light absorption, Then add a known concentration of isobutylpipe from the blank urine