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从玉米抗旱自交系CN165中克隆得到了与逆境胁迫相关的bZIP(Basic Leucine Zipper Protein)基因ZmbZIP71。Zm-bZIP71基因的开放阅读框为471bp,编码156个氨基酸,相对分子量为17.59 kDa,等电点pI为9.24。ZmbZIP71蛋白包含真核生物中高度保守的bZIP结构域。ZmbZIP71基因编码区的基因组包括2个外显子和1个内含子。利用实时荧光定量PCR方法分析ZmbZIP71基因在玉米不同组织中的表达差异及其在非生物胁迫下的表达模式,结果表明,该基因在玉米的多个组织和器官中均有表达,其中在雄穗和雌穗中的表达量较高。另外,该基因受干旱、低温和ABA的胁迫诱导上调表达,受盐胁迫下调表达。因此推测ZmbZIP71基因可能在玉米的逆境胁迫响应过程中具有重要作用。
The bZIP (Basic Leucine Zipper Protein) gene ZmbZIP71 was cloned from the maize drought-tolerant inbred line CN165. The open reading frame of the Zm-bZIP71 gene was 471 bp, encoding a protein of 156 amino acids with a relative molecular mass of 17.59 kDa and an isoelectric point pi of 9.24. The ZmbZIP71 protein contains a highly conserved bZIP domain in eukaryotes. The genome of ZmbZIP71 gene coding region includes two exons and one intron. The expression of ZmbZIP71 in different tissues of maize and its expression pattern under abiotic stresses were analyzed by real-time fluorescence quantitative PCR. The results showed that the gene was expressed in many tissues and organs of maize, And female ear higher expression. In addition, the gene was up-regulated by drought, hypothermia and ABA stress and down-regulated by salt stress. Therefore, it is speculated that ZmbZIP71 may play an important role in the response of maize to stress.