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目的探讨黄芪百合颗粒对常压缺氧损伤小鼠脑组织的保护作用及其初步机制。方法将70只昆明种雄性小鼠随机分为空白对照组、阳性对照组、黄芪百合颗粒低、中、高[1.75、3.50、7.00g·(kg·d-1]]剂量组。常规饲养3d后灌胃给药,每日1次,连续30d。进行小鼠常压耐缺氧实验,检测小鼠缺氧存活时间,采用比色分析法测定脑组织中超氧化物歧化酶(T-SOD)、还原型谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-PX)及过氧化氢酶(CAT)水平,HE染色观察各组小鼠脑组织的病理形态学变化。结果与空白对照组比较,阳性对照组及黄芪百合颗粒组小鼠缺氧存活时间明显延长,脑组织中TSOD、GSH、GSH-PX及CAT水平明显升高(P<0.05或P<0.01),各组小鼠脑组织病理形态学未见明显改变。结论黄芪百合颗粒对常压缺氧损伤小鼠的脑组织具有良好保护作用,这与体内抗氧化酶系的作用有直接关系。
Objective To investigate the protective effect and its primary mechanism of Astragalus and Lily Granule on brain tissue of hypoxia-injured mice. Methods Seventy Kunming male mice were randomly divided into blank control group, positive control group, Astragalus membranaceus Lily granules with low, medium and high doses of [1.75,3.50,7.00 g · kg -1] After intragastric administration, once daily for 30 days, the mice were subjected to hypoxia tolerance test, the hypoxia survival time was measured, and the content of superoxide dismutase (T-SOD) , Glutathione (GSH), glutathione peroxidase (GSH-PX) and catalase (CAT) were measured by enzyme-linked immunosorbent assay (ELISA). The pathological changes of brain tissue were observed by HE staining.Results Compared with the blank control group, the survival time of hypoxia in the positive control group and Astragalus-Lily Granules group was significantly prolonged, and the levels of TSOD, GSH, GSH-PX and CAT in the brain tissue were significantly increased (P <0.05 or P <0.01) There was no significant change in the histopathology of the mice.Conclusion Astragalus and Lily granules have a good protective effect on the brain tissue of mice under hypoxic conditions of normal pressure, which is directly related to the role of antioxidant enzymes in vivo.