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目的 筛选冻干乙脑活疫苗的保护剂。方法 通过优化组合筛选出A、C、D、E、F 5种保护剂配方 ,其中A为现行生产配方作为对照与其他配方进行比较。结果 使用A保护剂的疫苗在 37℃放置 7d和 2 1d ,病毒滴度分别下降 0 .98和 1.6 6LogPFU/ml;使用D、E保护剂的疫苗热稳定性最好 ,在 37℃放置 7d滴度分别下降 0 .19和 0 .0 7LogPFU/ml,2 1d分别下降 0 .42和 0 .39logPFU/ml。结论 D、E保护剂配方为最佳候选配方
Objective To screen the protective agent of lyophilized JN live vaccine. Methods Five kinds of protective agent formulations of A, C, D, E and F were screened out by optimized combination, among which A was compared with other formulations as the current production formula. Results The vaccine with A protectant was stored at 37 ° C for 7d and 21d and the virus titers dropped by 0.98 and 1.6 6LogPFU / ml, respectively. The vaccine with D and E protectors had the best thermostability, and the 7d drop Degrees decreased by 0.19 and 0.07LogPFU / ml respectively, and decreased by 0.42 and 0.39logPFU / ml respectively at 21d. Conclusion D, E protectant formulations for the best candidate formula