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目的：观察预防性给与神经节苷脂对大鼠坐骨神经切断后华勒氏变性的影响，并对其机制进行研究。方法：连续８ｄ给大鼠ｉｐ５０和８０ｍｇ·ｋｇ－１·ｄ－１神经节苷脂，第９天将大鼠坐骨神经切断，切断后分别于第７天，第１１天和第１６天用图象分析仪检查该神经远段内有髓神经纤维变性情况。结果：无论于第７天还是于第１１天观察，坐骨神经内均出现完整髓鞘神经纤维，而生理盐水对照组神经纤维全部崩解。前者各自的第７天组坐骨神经内神经纤维数均比第１１天组多，有显著性差异（Ｐ＜０．００１）；８０ｍｇ·ｋｇ－１组坐骨神经内神经纤维数与５０ｍｇ·ｋｇ－１组相比，无论于第７天还是于第１１天均无显著性差异（Ｐ＞０．０５）。以上各组神经纤维轴突直径，神经纤维直径以及它们之间的比值均与正常坐骨神经内有髓神经纤维相似（Ｐ＞０．０５）。无论是５０ｍｇ·ｋｇ－１组还是８０ｍｇ·ｋｇ－１组，于第１６天坐骨神经内均无完整髓鞘神经纤维。坐骨神经切断前后均用５０ｍｇ·ｋｇ－１，各组于切断后第７天、第１１天和第１６天坐骨神经内均保留有完整髓鞘神经纤维，其中第７天组与只在损伤前给５０ｍｇ·ｋｇ－１和８０ｍｇ·ｋｇ－１第７天两组相比无显著性差异（Ｐ＞０．０５）；而第１１?
Objective: To observe the effects of prophylactic administration of gangliosides on the Walerian degeneration after sciatic nerve transection in rats and to study its mechanism. Methods: Rats were given ip and 80 mg · kg-1 · d-1 gangliosides for 8 days. The sciatic nerve of rats was cut off on the 9th day. After the cut-off, the rats were sacrificed on days 7, 11 and 16 respectively Analyzer to check the nerve distal myelinated nerve fiber degeneration. Results: No matter on the 7th day or the 11th day, the intact myelinated nerve fibers appeared in the sciatic nerve, while the nerve fibers in the saline control group all collapsed. The number of nerve fibers in the sciatic nerve on the 7th day in each group was significantly higher than that on the 11th day (P <0.001). The number of nerve fibers in the sciatic nerve of 80 mg · kg-1 group was significantly higher than that in the group of 50 mg · kg-1 There was no significant difference between the 7th day and the 11th day (P> 0.05). The axonal diameter, nerve fiber diameter and the ratio between them in the above groups were similar to those in normal sciatic nerve (P> 0.05). No matter whether it was 50 mg · kg-1 or 80 mg · kg-1, there was no complete myelinated nerve fibers in the sciatic nerve on the 16th day. Before and after sciatic nerve transection were used 50mg · kg-1, in each group on the 7th, 11th and 16th day after the sciatic nerve retained intact myelinated nerve fibers, of which the first 7 days and only before injury to 50mg · There was no significant difference between the two groups on day 7 at kg-1 and 80 mg · kg-1 (P> 0.05)