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最初认为TNF-α是由单核巨噬细胞产生。近来有研究证明,小鼠及兔子的肥大细胞及一些体外传代培养的肥大细胞系均具有合成及分泌TNF-α的能力。作者等应用L929细胞毒试验,测定经声波破坏细胞后上清中TNF-α活性,发现经分离纯化小鼠腹腔肥大细胞(纯度为91～94%)可表达较高
TNF-a was originally thought to be produced by mononuclear macrophages. Recently, studies have shown that mouse and rabbit mast cells and some of the in vitro cultured mast cell lines have the ability to synthesize and secrete TNF-α. The authors applied the L929 cytotoxicity assay to determine the activity of TNF-α in the supernatant after sonicated cells, and found that peritoneal mast cells (with purity of 91-94%) could be expressed higher