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目的探讨ATⅡ受体1(AGTR1)和二肽肌肽酶1(CNDP1)基因多态性与糖尿病慢性肾脏疾病(CKD)的相关性。方法选取T2DM患者292例,采集外周血标本,全血用于提取基因组DNA,血清用于检测生化指标。利用聚合酶链反应-限制性片段长度多态性分析法(PCR-RFLP)测序分析AGTR1和CNDP1基因多态性。结果 AGTR1的C等位基因是CKD的危险因素(P=0.028,OR=2.269,95%CI:1.134~6.837),AGTR1基因rs5186基因多态性可能为患CKD危险因素。CNDP1的C等位基因是CKD的危险因素(P=0.024,OR=2.571,95%CI:1.127~4.211),CNDP1基因rs4892247基因多态性可能为患CKD的危险因素。结论 AGTRl的C等位基因和CNDP1的C等位基因与CKD相关。
Objective To investigate the relationship between the polymorphism of ATⅡ receptor 1 (AGTR1) and dipeptidyl peptidase 1 (CNDP1) and chronic kidney disease (CKD) in diabetic patients. Methods Totally 292 T2DM patients were selected to collect peripheral blood samples. Whole blood was used to extract genomic DNA and serum was used to detect biochemical parameters. The polymorphisms of AGTR1 and CNDP1 were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results AGTR1 C allele was a risk factor for CKD (P = 0.028, OR = 2.269, 95% CI: 1.134-6.837). AGTR1 rs5186 gene polymorphism may be a risk factor for CKD. C allele of CNDP1 was a risk factor for CKD (P = 0.024, OR = 2.571, 95% CI: 1.127-4.211). The SNP rs4892247 gene polymorphism may be a risk factor for CKD. Conclusions The C allele of AGTR1 and C allele of CNDP1 are associated with CKD.