【摘 要】
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AIM: To investigate the pathological effect of Helicobacter pylori(H pylori) on human hepatic ceils, proteomic methods were used to find and to identify protein
【机 构】
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Department of Infectious Diseases,Medical Research Center
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AIM: To investigate the pathological effect of Helicobacter pylori(H pylori) on human hepatic ceils, proteomic methods were used to find and to identify proteins that were overexpressed in HepG2 cells treated by H pylori.METHODS: H pylori was co-cultured with HepG2 for 6 h. Two-dimensional gel electrophoresis was used to gainthe protein expression pattern of untreated and H pyloritreated HepG2. After staining and image analysis,spots of interest were isolated and subjected to massspectrometry.RESULTS: Seven proteins, which were up-regulated inH p ylori-treated HepG2 cells, were identified. These proteinsincluded integrin beta-1, protein kinase C alpha, LIM/ homeobox protein Lhx1, eIF-2-beta, MAP kinase kinase 3, PINCH protein and Ras-related protein Rab-37, which involved in transcription regulation, signal transduction, metabolism and so on.CONCLUSION: H pylori may exert the pathological effect on HepG2 cells by up-regulating the expression of some proteins.
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