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该文对毛白杨幼苗在低温锻炼中钙调蛋白 (CaM)、6 磷酸葡萄糖脱氢酶 (G6PDHase)、腺苷三磷酸酶 (ATPase)、幼苗存活率及抗冻性的动态变化过程进行了测定 .结果表明 ,为了获得较高的抗冻性 ,生长期毛白杨幼苗的低温锻炼可分 3阶段进行 ,其中第 3阶段的 - 3℃锻炼对毛白杨幼苗抗冻性发育最有效 ;低温锻炼提高了幼苗叶片和枝条中CaM含量和G6PDHase及ATPase活性 ,同时也提高了幼苗的存活率和抗冻性 ,但毛白杨幼苗叶片的CaM含量和G6PDHase及ATPase活性提高程度较枝条明显 .进一步分析发现 ,在 - 3℃低温锻炼期间 ,上述各项指标变化与幼苗抗冻性的提高存在着明显的相关性 ,CaM可能参与G6PDHase和ATPase活性的调节 .
The dynamic changes of calmodulin (CaM), G6PDHase, ATPase, seedling survival rate and frost resistance in Populus tomentosa seedlings during cold acclimation were measured The results showed that in order to obtain higher frost resistance, the cold temperature training of Populus tomentosa seedlings can be divided into three stages, of which the third stage - 3 ℃ exercise is the most effective frost resistance development of Populus tomentosa seedlings; The content of CaM, G6PDHase and ATPase activities in seedling leaves and shoots, as well as the survival rate and frost resistance of seedlings were increased, but the content of CaM and G6PDHase and ATPase activities in the leaves of Populus tomentosa seedlings increased more obviously than that of shoots.Further analysis showed that, During low temperature exercise at -3 ℃, there was a significant correlation between these changes and the improvement of frost resistance of seedlings. CaM may be involved in the regulation of G6PDHase and ATPase activities.