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目的通过对新疆艾滋病病毒(HIV)感染者的病毒基因序列分析,了解2010-2013年新疆HIV亚型和耐药传播情况。方法采集当年新报告的16~25岁未接受抗病毒治疗的HIV-1感染者的血浆样本,提取病毒核糖核酸(RNA),通过扩增HIV-1 pol区片段,进行测序和耐药基因变异分析。结果 4年共获得pol区序列150条,主要以CRF07_BC为主(96.7%,145/150),共有3例出现耐药,分别针对非核苷类抑制剂(NNRTIs)和核苷类抑制剂(NRTIs)。耐药株主要出现在性传播人群中。结论各传播途径中仍主要流行CRF07_BC,新疆HIV-1耐药传播目前处于低度传播水平,但仍需进一步加强对感染者的耐药监测和病人的治疗管理。
Objective To understand the HIV subtypes and drug-resistant transmission in Xinjiang from 2010 to 2013 through the sequence analysis of the virus genes of HIV-infected people in Xinjiang. Methods The newly reported plasma samples from 16-25-year-old HIV-1-infected patients who did not receive anti-virus treatment were collected and viral RNA was extracted. The HIV-1 pol region was amplified and sequenced. analysis. Results A total of 150 pol region sequences were obtained in 4 years, mainly CRF07_BC (96.7%, 145/150). A total of 3 cases were resistant to NNRTIs and NRTIs ). Resistant strains mainly appear in sexually transmitted infections. Conclusion CRF07_BC is still predominant in all routes of transmission. The transmission of drug-resistant HIV-1 in Xinjiang is currently at a low level of transmission, but drug resistance surveillance and patient management are still needed to be further strengthened.