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目的:研究多肽铬螯合的对糖尿病小鼠肝脏蛋白质表达的影响,探讨其治疗糖尿病的机理。方法:通过腹腔注射四氧嘧啶建立糖尿病小鼠模型。将小鼠分为正常组、模型组和胶铬组,胶铬组以灌胃方式加入多肽铬螯合物;以光镜及 HE 染色观察三组小鼠肝脏形态及组织学的变化,采用 SDS-PAGE 实验和非 SDS-PAGE 检测三组小鼠肝脏蛋白质表达。结果:光镜及组织学观察结果显示多肽铬螯合物可以有效地减轻四氧嘧啶对肝细胞造成的损伤。模型组肝脏 25kDa-35kDa 之间的某种蛋白表达升高而多肽铬螯合物可以降低此种蛋白的表达,初步推断这种蛋白质为 SOD。结论:多肽铬螯合物能够通过降低四氧嘧啶造成的肝脏中抗氧化有关的蛋白质代偿性升高而祈祷保护肝脏的作用,是一种新型的治疗糖尿病所致的肝损伤的活性物质。
OBJECTIVE: To study the effect of chymotrypsin chelating on liver protein expression in diabetic mice and to explore its mechanism of diabetes mellitus. Methods: Diabetic mice model was established by intraperitoneal injection of alloxan. The mice were divided into normal group, model group and chromate group. Chromium chondrocyte group was fed with chrome chelate by gavage. The morphological and histological changes of liver were observed by light microscope and HE staining. The protein expression of liver in the three groups was detected by-PAGE and non-SDS-PAGE. Results: Light microscopy and histological observation showed that the polypeptide chrome chelate could effectively reduce the damage caused by alloxan to hepatocytes. In the model group, the expression of a certain protein was increased between 25kDa-35kDa in the liver and the polypeptide chrome chelate could reduce the expression of this protein. It was preliminarily concluded that this protein was SOD. CONCLUSION: Peptide chrome chelators can pray for the protection of the liver by reducing the compensatory increase of anti-oxidation-related proteins in the liver caused by alloxan. It is a novel active substance for treating liver damage caused by diabetes.