论文部分内容阅读
在0.01 mol·L-1Hepes,0.15 mol·L-1Na Cl,p H 7.4及室温条件下,采用紫外差光谱和荧光光谱的方法研究了稀土离子Ho(III),Er(III),Yb(III)与八肋游仆虫中心蛋白N端半分子(N-terminal domain of Euplotes octocarinatus centrin,N-Eo Cen)的结合性质。结果表明:稀土离子Ho(III),Er(III),Yb(III)与N-Eo Cen结合导致蛋白质高级结构发生明显变化,疏水区暴露增加。二甲酚橙为竞争配体测得稀土离子Ho(III),Er(III),Yb(III)与N-Eo Cen两个位点的结合能力分别为:lg KI,Ho(III)=5.47±0.37,lg KII,Ho(III)=5.07±0.45;lg KI,Er(III)=5.78±0.42,lg KII,Er(III)=5.36±0.52;lg KI,Yb(III)=5.80±0.65,lg KII,Yb(III)=5.37±0.61。
The effects of rare earth ions Ho (III), Er (III), Yb (III) and their salts on the luminescence of rare earth ions were studied by using UV spectra and fluorescence spectroscopy in 0.01 mol·L-1Hepes, 0.15 mol·L-1 NaCl and pH 7.4. ) And the N-terminal domain of Euplotes octocarinatus centrin (N-Eo Cen). The results showed that the combination of rare earth ions Ho (III), Er (III), Yb (III) and N-Eo Cen resulted in significant changes in the protein structure and increased hydrophobic region exposure. Xylenol orange is a competitive ligand. The binding capacities of rare earth ions Ho (III), Er (III), Yb (III) and N-Eo Cen are respectively lg KI and Ho (III) = 5.47 ± 0.37, lg KII and Ho (III) = 5.07 ± 0.45; lg KI and Er (III) = 5.78 ± 0.42, lg KII and Er (III) = 5.36 ± 0.52; , lg KII, Yb (III) = 5.37 ± 0.61.