用超滤、硫酸铵二段盐析法取代等电点沉淀法后，精制破伤风类毒素（精破类）的纯度由８０７Ｌｆ／ｍｇＰＮ提高到１８８３Ｌｆ／ｍｇＰＮ，纯度提高一倍以上。使用双胨培养基取代酪素培养基后，产毒水平由４７Ｌｆ／ｍｌ提高到８８Ｌｆ／ｍｌ（ｔ＝６．４６，ｐ＜０．００１）；用新法精制后，精破类纯度分别为１９４９Ｌｆ／ｍｇＰＮ及１７８５Ｌｆ／ｍｇＰＮ（ｔ＝０．３３４，ｐ＞０．０５），引用双胨培养基后可提高产毒水平，但不影响精破类的纯度。 Purification of tetanus toxoid (broken type) by ultrafiltration and ammonium sulfate second-stage salting out instead of isoelectric point precipitation increased the purity from 807 Lf / mgPN to 1883 Lf / mgPN, and the purity was doubled. After using peptone medium to replace the casein medium, the level of toxin production increased from 47Lf / ml to 88Lf / ml (t = 6.46, p <0.001) / MgPN and 1785Lf / mgPN (t = 0.334, p> 0.05). The reference to the peptone medium can increase the level of toxin production, but does not affect the purity of the broken class.