本研究旨在用玉米未成熟雄花序作为外植体,以获得接近正常发育的小穗。由于供培养的雄穗易受影响,而且脱离了植株其余器官的影响,因此可以进行许多以前难于进行或不可能进行的研究。例如使正常雄穗生长和分化所必需的营养因子的研究成为可能,它们是减数分裂和小孢子发生的基础生化研究。此外,还可能进行诸如诱导花药单倍体和用化学方法使 The purpose of this study is to use immature male inflorescences as explants to obtain spikelets near normal development. Because of the susceptibility of cultured tassels to spikes and the impact of the remaining organs of the plant, many previously difficult or impossible studies have been conducted. For example, the study of trophic factors necessary for the growth and differentiation of normal tassels has become possible, and they are fundamental biochemical studies of meiosis and microsporogenesis. In addition, it is also possible to perform, for example, induction of anther haploid and use of chemical methods