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目的应用ELISA法检测丙型肝炎核心抗原(HCV-cAg),探讨HCV-cAg在丙型肝炎早期诊断中的临床价值。方法用ELISA法联合检测858例临床手术前和输血患者HCV-cAg和抗-HCV。对其中29例联合检测阳性患者再进行核酸(HCV-RNA)检测,结果进行比较分析。结果联合检测HCV-cAg和抗-HCV,HCV-cAg阳性率为0.93%,抗-HCV阳性率为2.80%,联合检测阳性率为3.38%。与金标准HCV-RNA检测比较,HCV-cAg检测敏感性为87.50%,特异性为95.24%,准确度为93.10%,两种方法检测结果差异无统计学意义(P>0.05),具有可比性。结论联合检测HCV-cAg和抗-HCV,提高了丙型肝炎病毒感染者检出的阳性率,减少了丙型肝炎病毒感染者的漏检。同时HCV-cAg检测操作简便,经济快捷,与HCV-RNA检测一致性高,有效的缩短了窗口期,值得临床推广应用。
Objective To detect the hepatitis C core antigen (HCV-cAg) by ELISA and to explore the clinical value of HCV-cAg in the early diagnosis of hepatitis C. Methods Enzyme linked immunosorbent assay (ELISA) was used to detect HCV-cAg and anti-HCV in 858 preoperative and transfusion patients. Of 29 patients with positive joint detection of nucleic acid (HCV-RNA) detection, the results were compared. Results The positive rates of HCV-cAg and HCV-cAg were 0.93% and 2.80%, respectively. The positive rate of HCV-cAg and anti-HCV was 3.38%. Compared with the gold standard HCV-RNA test, the detection sensitivity and specificity of HCV-cAg were 87.50%, 95.24% and 93.10%, respectively. There was no significant difference between the two methods (P> 0.05) . Conclusions The combined detection of HCV-cAg and anti-HCV can improve the positive rate of hepatitis C virus infection and reduce the missed detection of hepatitis C virus infection. At the same time HCV-cAg detection is simple, economical and efficient, and HCV-RNA detection of high consistency, effectively shorten the window period, it is worthy of clinical promotion and application.