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目的:探讨心力衰竭患者外周血单核细胞端粒长度和端粒酶h TERT活性在心衰发生进程中的变化情况和意义。方法:按照筛选要求选择患者,根据入选标准分为心衰组(49例)和非心衰组(44例)。记录患者的年龄、性别、生活习惯及疾病情况,超声检测患者心脏功能,测量左心室舒张末内径(LVEDD)、左心室射血分数(LVEF)。在不同时间点,抽取外周血分离单核细胞,用PCR方法检测端粒长度和端粒酶h TERT活性。结果:对照组比较,心衰组患者心脏左室舒张末内径明显增加,射血分数明显降低(P<0.05);在第1、7天,心衰组患者外周血单核细胞端粒长度较对照组明显缩短、端粒酶h TERT活性明显增强。第7天较同组第1天端粒长度有所增加,端粒酶h TERT活性有所减低,但与对照组相比,端粒长度显著缩短,端粒酶h TERT显著增高(P<0.05)。结论:心力衰竭后患者端粒长度和端粒酶h TERT活性明显变化,并随心衰发病具有一定波动,提示端粒和端粒酶可能参与了心衰发展进程。
Objective: To investigate the changes of telomere length and telomerase h TERT activity in peripheral blood mononuclear cells during heart failure in patients with heart failure. Methods: The patients were selected according to the screening criteria. According to the criteria, the patients were divided into heart failure group (n = 49) and non-heart failure group (n = 44). The patient’s age, sex, lifestyle and disease were recorded. Cardiac function was measured by ultrasonography. Left ventricular end-diastolic diameter (LVEDD) and left ventricular ejection fraction (LVEF) were measured. At different time points, mononuclear cells were extracted from peripheral blood and telomere length and telomerase h TERT activity were detected by PCR. Results: Compared with the control group, the left ventricular end-diastolic diameter increased significantly and the ejection fraction decreased significantly (P <0.05). On the first day, the telomere length of peripheral blood mononuclear cells The control group was significantly shortened, hTERT activity was significantly enhanced. Compared with the control group, the telomere length was significantly shortened and telomerase h TERT was significantly increased (P <0.05). On the 7th day, ). CONCLUSION: The telomere length and the telomerase h TERT activity in patients with heart failure significantly change and fluctuate with the progression of heart failure, suggesting that telomeres and telomerase may be involved in the development of heart failure.