目的:研究紫草素对人卵巢癌SKOV3细胞的影响,并对其机制进行初步研究。方法:以不同浓度的紫草素干预人卵巢癌SKOV3细胞,采用MTT法观察紫草素对肿瘤细胞增殖的影响;流式细胞术检测经紫草素干预48 h后SKOV3细胞的凋亡情况和细胞周期,并以Western blot法测定Bax及Bcl-2蛋白表达。结果:紫草素(0.5,1.0,2.0,4.0,8.0,16.0μg/ml)经过24 h,48 h及72 h对肿瘤细胞增殖的抑制率分别为6.90%,12.11%,20.82%,30.20%,41.69%,56.81%;7.28%,12.16%,20.18%,40.81%,51.23%,70.51%;10.50%,15.90%,28.16%,46.86%,59.01%,78.58%,呈剂量依赖性和时间依赖性的关系。流式细胞术检测结果显示,人卵巢癌SKOV细胞经紫草素(3.0,6.0,12.0μg/ml)处理48 h后,其凋亡率分别为20.58%,35.10%及51.26%,G0/G1期细胞的比例分别62.10%,63.91%及83.86%。Western blotting检测结果显示,人卵巢癌SKOV细胞经紫草素(3.0,6.0,12.0μg/ml)处理48 h后,Bcl-2蛋白水平表达随紫草素浓度递增减少,而Bax蛋白水平随紫草素浓度递增而递增。结论:紫草素可抑制人卵巢癌细胞SKOV-3的体外增殖并诱导其凋亡,其机制可能为上调Bax表达和下调Bcl-2表达有关。 Objective: To study the effect of shikonin on human ovarian cancer cell line SKOV3 and its mechanism. METHODS: Human ovarian cancer SKOV3 cells were treated with different concentrations of shikonin. The effects of shikonin on the proliferation of SKOV3 cells were observed by MTT assay. The apoptosis of SKOV3 cells treated with shikonin for 48 h was measured by flow cytometry. Cell cycle and Western blot were used to determine the protein expression of Bax and Bcl-2. Results: The inhibitory rates of shikonin (0.5,1.0,2.0,4.0,8.0,16.0μg / ml) on the proliferation of tumor cells after 24 h, 48 h and 72 h were 6.90%, 12.11%, 20.82%, 30.20% , 41.69%, 56.81%, 7.28%, 12.16%, 20.18%, 40.81%, 51.23%, 70.51%, 10.50%, 15.90%, 28.16%, 46.86%, 59.01%, 78.58% in a dose- and time- Sexual relations. Flow cytometry showed that the apoptotic rates of SKOV cells were 20.58%, 35.10% and 51.26%, respectively, after treatment with shikonin (3.0, 6.0, 12.0μg / ml) The proportion of stage cells were 62.10%, 63.91% and 83.86% respectively. Western blotting results showed that the expression of Bcl-2 protein in SKOV cells treated with shikonin (3.0, 6.0, 12.0 μg / ml) for 48 h decreased with the increase of shikonin concentration, while the level of Bax protein increased with purple Hormone concentration increased with increasing. Conclusion: Shikonin can inhibit the proliferation and induce the apoptosis of human ovarian cancer cell line SKOV-3 in vitro. The mechanism may be related to the up-regulation of Bax expression and down-regulation of Bcl-2 expression.