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目的:探讨雄激素受体(AR)在前列腺癌雄激素依赖特性转化过程中的作用。方法:对33例晚期前列腺癌患者进行雄激素阻断治疗并作长时间随访,其间有18例发生了雄激素依赖转化,15例未发生雄激素依赖转化。采用免疫组织化学及RT-PCR法测定18例患者雄激素依赖转化前后及15例患者雄激素阻断治疗前后癌细胞内AR蛋白及AR基因的表达情况。结果:18例患者雄激素依赖转化前后AR蛋白及AR基因的表达分别为(1.33±0.97 vs 3.11±0.76)和(28.41±3.38Ct vs 36.73±1.81Ct),两者之间差异有统计学意义(P<0.01);15例患者雄激素阻断治疗前后AR蛋白及AR基因的表达分别为(1.47±0.83 vs 1.40±0.99)和(29.50±3.08Ct vs 29.14±3.23Ct),两者之间差异无统计学意义(P>0.05)。结论:AR基因及AR蛋白表达增强是前列腺癌雄激素依赖转化的原因之一。
Objective: To investigate the role of androgen receptor (AR) in the process of androgen-dependent transformation of prostate cancer. METHODS: Forty-three patients with advanced prostate cancer were treated with androgen blockade for a long period of follow-up. During this period, 18 patients underwent androgen-dependent transformation and 15 patients did not undergo androgen-dependent transformation. Immunohistochemistry and RT-PCR were used to determine the expression of AR and AR in 18 cases before and after androgen-dependent transformation and before androgen treatment in 15 cases. Results: The expression of AR protein and AR gene in androgen-dependent manner in 18 patients were (1.33 ± 0.97 vs 3.11 ± 0.76) and (28.41 ± 3.38Ct vs 36.73 ± 1.81Ct), respectively, with significant difference between the two (1.47 ± 0.83 vs 1.40 ± 0.99) and (29.50 ± 3.08 Ct vs 29.14 ± 3.23 Ct, respectively) before and after androgen ablation therapy in 15 patients (P <0.01) The difference was not statistically significant (P> 0.05). Conclusion: The increased expression of AR and AR proteins is one of the reasons for the androgen-dependent transformation of prostate cancer.