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目的:研究蒿甲醚及双氢青蒿素对小鼠硬皮病模型的影响。方法:随机将60只Balb/c小鼠分为正常组,模型组,溶媒组,阳性药组(青霉胺200mg·kg~(-1)),蒿甲醚低,高剂量组(5,20mg·kg~(-1)),双氢青蒿素低,高剂量组(5,25 mg·kg~(-1))。以0.1 mL 200 mg·L~(-1)博莱霉素对小鼠进行皮下注射,持续3周,制备硬皮病模型。造模同时灌胃给予不同剂量药物4周。给药完成后次日取皮肤和肺进行分析。观察HE染色后小鼠皮肤厚度和真皮纤维化程度,对皮肤羟脯氨酸含量,胶原含量进行测定。结果:与模型组相比,各药高剂量组皮肤厚度,胶原含量显著减少,其他各治疗组小鼠皮肤硬化程度也得到一定的改善。结论:蒿甲醚、双氢青蒿素对预防性治疗硬皮病小鼠有一定的疗效。
Objective: To study the effects of artemether and dihydroartemisinin on mouse scleroderma model. Methods: 60 Balb / c mice were randomly divided into normal group, model group, vehicle group, positive drug group (penicillamine 200 mg · kg -1), artemether low and high dose group (5, 20 mg · kg -1), dihydroartemisinin low and high dose group (5, 25 mg · kg -1). The mice were injected subcutaneously with 0.1 mL 200 mg · L -1 bleomycin for 3 weeks to prepare a scleroderma model. Simultaneous intragastric administration of different doses of drugs for 4 weeks. The next day after administration, skin and lungs were taken for analysis. The thickness of mouse skin and the degree of dermal fibrosis were observed after HE staining, and the contents of hydroxyproline and collagen in the skin were measured. Results: Compared with the model group, the skin thickness and the collagen content of the high dose group of each drug were significantly reduced, and the degree of skin sclerosis of the other treatment groups was also improved. Conclusion: Artemether and dihydroartemisinin have some effect on the preventive treatment of scleroderma in mice.