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为探索兰花花器官形成机理,我们从莲瓣兰花朵转录组测序的MADS基因家族成员中,筛选出8个基因,并用MEGA6.0和MEME软件对其生物信息学进行分析,并进行了相对表达量分析。研究结果表明,8条MADS基因分别与其它物种MADS-box家族成员有较高同源性。MADS-box结构域分析显示,除了Unigene0032261只拥有M结构外,所有基因都拥有M,I,K,C结构域。荧光定量PCR分析表明,这8个基因,可能与花器官的发育相关,参与了除鼻头外,花器官不同组织部位的发育,在营养器官中也检测到不同程度的表达。Unigene0024850、Unigene0024850、Unigene0032261、Unigene0013190、Unigene0026604与Unigene0019639在唇瓣中也不同程度表达,而Unigene0024956和Unigene0021946在唇瓣中表达最低,推测前六个基因可能参与了唇瓣的发育,而后者2个对唇瓣发育作用不大。其中Unigene0025650在小舌中高表达,可能参与了多舌的形成。本试验为莲瓣兰花发育及多舌奇花形成机理提供了一定的理论依据。
To explore the organogenesis mechanism of orchid flower, 8 genes were screened from members of the MADS gene family sequenced by Lantana orchid transcriptome and analyzed by bioinformatics using MEGA6.0 and MEME software, and relative expression Quantitative analysis. The results showed that eight MADS genes have high homology with MADS-box family members of other species respectively. MADS-box domain analysis revealed that all genes possess M, I, K, C domains, except that Unigene0032261 has only the M structure. Fluorescent quantitative PCR analysis showed that these 8 genes may be involved in the development of floral organs, involved in the development of different tissues of floral organs except for the nose, and also expressed different levels in vegetative organs. Unigene0024850, Unigene0024850, Unigene0032261, Unigene0013190, Unigene0026604 and Unigene0019639 were expressed in different degrees in the lip, whereas Unigene0024956 and Unigene0021946 had the lowest expression in the lip, suggesting that the first six genes may be involved in the development of the lip, while the latter two pairs Little development of the flap. Among them, Unigene0025650 is highly expressed in the small tongue and may be involved in the formation of multiple tongues. This experiment provides some theoretical basis for the development of lotus orchid and the formation mechanism of many tongue-blossoming flowers.