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目的:评价检测淋巴细胞检测淋巴细胞HLA-B27抗原在强直性脊柱炎(ankylosingspondylitis,AS)患者、腰痛患者及健康人中的表达及临床意义。方珐:取肘静脉血Iml(EDTANa2抗凝),采用荧光抗体HLA-B27-FITC/HLAB7-PE直接标记法,流式细胞仪检测淋巴细胞中HLA-B27的比例。结果:24例AS患者、35例腰痛患者和55例健康者中HLA-B27阳性率分别为91.67%、5.71%、5.45%。结论:HLA-B27与强直性脊柱炎高度相关,其检测是AS诊断与鉴别诊断的重要标志。但检测阳性并不能作为AS的确诊和筛选指标;流式细胞仪检测HLA-B27简便省时,稳定性和重复性好,适用于临床检验。
OBJECTIVE: To evaluate the expression and clinical significance of HLA-B27 antigen detected by lymphocyte detection in patients with ankylosponsive spinal cord inflammation (AS), lumbago and healthy subjects. Fang enamel: elbow vein blood Iml (EDTANa2 anticoagulant), using fluorescent antibody HLA-B27-FITC / HLAB7-PE direct labeling method, flow cytometry detection of HLA-B27 ratio in lymphocytes. Results: The positive rates of HLA-B27 in 24 AS patients, 35 low back pain patients and 55 healthy controls were 91.67%, 5.71% and 5.45% respectively. Conclusion: HLA-B27 is highly correlated with ankylosing spondylitis, and its detection is an important marker for the diagnosis and differential diagnosis of AS. However, positive detection can not be used as a diagnostic and screening index for AS. The detection of HLA-B27 by flow cytometry is simple, time-saving, good stability and repeatability, and is suitable for clinical examination.