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水稻簇矮病毒（ＲＢＳＶ）是由黑尾叶蝉（Ｎｅｐｈｏｔｅｔｉｘｃｉｎｃｔｉｃｅｐｓ）和二点黑尾叶蝉（Ｎ．ｖｉｒｅｓｃｅｎｓ）传播的持久性病毒，但不经卵传递；病害表现有奇特的多蘖症或节枝症，且其病株汁液与水稻矮缩病毒（ＲＤＶ）抗血清不起免疫反应．采用差速离心和蔗糖密度梯度离心，可获得ＲＢＳＶ提纯制剂，提纯制剂经紫外扫描，呈典型的核蛋白吸收曲线，Ａ２６０／２８０＝１．６４．在电镜下观察提纯病毒，可见大量比较均一的球状粒体，直径为６０．０ｎｍ．病毒粒体有双层衣壳，蛋白亚基清晰可辨．通过免疫扩散和免疫电镜试验，病毒粒体与ＲＢＳＶ抗血清有明显的血清学反应，而这种抗血清与ＲＤＶ、水稻瘤矮病毒（ＲＧＤＶ）不起反应．提纯ＲＢＳＶ制剂以苯酚－甲基苯酚－ＳＤＳ方法提取核酸．将其注射到昆虫介体内，具有侵染性．核酸制品有典型的核酸紫外吸收曲线，Ａ２６０／２８０＝２．０２．根据核酸在不同离子强度下对ＲＮａｓｅ的稳定性等的测定，表明其核酸属双链ＲＮＡ．根据紫外吸收测定，ＲＮＡ在ＲＢＳＶ粒体中的含量为１７．５％．用聚丙烯酰胺凝胶电泳测定，其ＲＮＡ的总分子质量为１６．６６×１０６．各组分相对分子质量分别为２．７０×１０６、２．３０×１０６、１?
Rice cluster dwarf virus (RBSV) is a persistent virus that is transmitted by Nephotetix cincticeps and N. virescens but is not transmitted by eggs; the disease manifests as strange tillering or disease Strain, and its diseased juice and rice dwarf virus (RDV) antiserum not immune response. Using differential centrifugation and sucrose density gradient centrifugation, the RBSV purified preparation was obtained. The purified preparation was characterized by ultraviolet scanning and showed a typical nuclear protein absorption curve with A260 / 280 = 1.64. Observed by electron microscopy purification of the virus, showing a large number of relatively uniform spherical granules, a diameter of 60.0nm. Virus mitochondria double capsid protein subunits clearly identifiable. Through immunodiffusion and immunoelectron microscopy, virosomes showed obvious serological reaction with RBSV antiserum, which could not react with RDV and rice galldy dwarf virus (RGDV). Purification of RBSV Formulations Nucleic acids were extracted by the phenol-methylphenol-SDS method. It is injected into insect mediators and is infectious. Nucleic acid products have a typical UV absorbance curve, A260 / 280 = 2.02. The determination of the stability of the RNase under different ionic strength by the nucleic acid indicates that the nucleic acid is double-stranded RNA. The amount of RNA in the RBSV granules was 17.5% as determined by UV absorption. Using polyacrylamide gel electrophoresis, the total molecular mass of RNA was 16.66 × 106. The relative molecular mass of each component were 2.70 × 106, 2.30 × 106,