Eight ergostane type sterols and three their derivatives (one mono linoleate and two mono glucosides) were isolated from the ethyl acetate soluble fraction of the fungus Catathelasma imperiale . Two of them are novel compounds, namely 22 E ,24 R ergosta 7,22 diene 3 β ,5 α diol 6 β linoleate (1) and 22 E ,24 R ergosta 7,22 diene 3 β ,5 β ,6 α triol (5) with an uncommon cis fused A/B ring. Structures of these compounds were demonstrated on the basis of their chemical evidences and spectroscopic methods, especially 2D NMR techniques. Eight ergostane type sterols and three their derivatives (one mono linoleate and two mono glucosides) were isolated from the ethyl acetate soluble fraction of the fungus Catathelasma imperiale. Two of them are novel compounds, namely 22 E, 24 R ergosta 7,22 diene 3 Structures of these compounds were synthesized by using an uncommon cis-fused A / B ring. on the basis of their chemical evidences and spectroscopic methods, especially 2D NMR techniques.