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目的:建立HPLC法同时测定野老鹳草中没食子酸,原儿茶酸,柯里拉京,金丝桃苷,槲皮素的含量测定方法。方法:采用RP-HPLC法,Agilent TC-C18色谱柱(250 mm×4.6 mm,5μm),0.1%磷酸水溶液-乙腈为流动相梯度洗脱,流速0.8 m L/min,柱温25℃。结果:没食子酸、原儿茶酸、柯里拉京、金丝桃苷和槲皮素的线性范围为1.158~23.160 g·L-1(r=0.9999);0.039~0.792 g·L-1(r=0.9998);1.498~29.960 g·L-1(r=0.9999);0.423~8.460 g·L-1(r=0.9998);0.021~0.401 g·L-1(r=0.9997)。平均回收率为99.35%(RSD 1.3%);98.54%(RSD 1.5%);99.17%(RSD 0.8%);98.97%(RSD 1.6%);99.49%(RSD 2.1%)。结论:该含量测定的方法快速,准确,重复性好,可为野老鹳草药材的质量控制提供依据。
Objective: To establish a HPLC method for the simultaneous determination of gallic acid, protocatechuic acid, corilagin, hyperoside and quercetin in Geranium spp. Methods: RP-HPLC method was used on a Agilent TC-C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of 0.1% phosphoric acid and acetonitrile. The flow rate was 0.8 m L / min and the column temperature was 25 ℃. Results: The linear ranges of gallic acid, protocatechuic acid, corilagin, hyperoside and quercetin were 1.158-23.160 g · L -1 (r = 0.9999), 0.039-0.792 g · L -1 (r = 0.9998); 1.498-29.960 g · L -1 (r = 0.9999); 0.423- 8.460 g · L -1 (r = 0.9998); 0.021-0.401 g · L -1 (r = 0.9997). The average recovery was 99.35% (RSD 1.3%); 98.54% (RSD 1.5%); 99.17% (RSD 0.8%); 98.97% (RSD 1.6%); 99.49% (RSD 2.1%). Conclusion: The method of determination of content is rapid, accurate and reproducible. It can provide the basis for the quality control of Geranium spp.