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目的探究人体富血小板凝胶(PRG)对金黄色葡萄球菌的体外抗菌作用;对比观察不同浓度富血小板凝胶对金黄色葡萄球菌的体外抗菌作用。方法分别取20例健康志愿者外周静脉血30 mnl,制备不同浓度富血小板凝胶,血小板浓度依次为1.8×10~(12)/L(PRG_1组)、1.2×10~(12)/L(PRG_2组)、0.6×10~(12)/L(PRG_3组)。通过体外抗菌实验观察不同浓度PRG在甲氧西林敏感金黄色葡萄球菌(MSSA)、耐甲氧西林金黄色葡萄球菌(MRSA)溶液中孵育1、2、4、8、12、24 h后的抗菌效果。结果在孵育开始后,各浓度PRG菌落计数明显少于PBS对照组,PRG对MRSA和MSSA均有明显的抗菌作用(P<0.05),各浓度PRG抗菌作用均在孵育2 h后最明显,随后逐渐下降;同一时间PRG_1、PRG_2和PRG_3的抑菌率依次下降,与其含血小板浓度呈正相关关系,各组间菌落计数差异均有统计学意义(P<0.05)。在孵育12 h后,细菌生长进入稳定期,各组抑菌率明显下降,各组菌落计数差异无统计学意义(P>0.05)。结论在一定时间内,PRG对MSSA和MRSA均有明显的抑制作用,PRG的抗菌作用与血小板浓度呈正相关关系。
Objective To investigate the in vitro antibacterial effect of human platelet-rich plasma (PRG) against Staphylococcus aureus and to compare the in vitro antibacterial activity of different concentrations of platelet-rich gel against Staphylococcus aureus. Methods Peripheral venous blood of 20 healthy volunteers was collected for 30 mnl to prepare platelet-rich gels of different concentrations. The platelet concentrations were in the order of 1.8 × 10 12 / L (PRG 1 group) and 1.2 × 10 12 / L PRG_2 group), 0.6 × 10 ~ (12) / L (PRG_3 group). Antibacterial activity of PRG in different concentrations of PRG in methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA) effect. Results At the beginning of incubation, the counts of PRG colonies were significantly less than those of PBS control group. PRG had obvious antimicrobial activity against both MRSA and MSSA (P <0.05). The antibacterial activities of PRG at different concentrations were most obvious after 2 h of incubation. (P <0.05). The inhibitory rates of PRG_1, PRG_2 and PRG_3 were decreased at the same time, which showed a positive correlation with the platelet-containing concentration. The difference of colony counts among the groups was statistically significant (P <0.05). After 12 h of incubation, the bacteria grew into a stable phase, and the antibacterial activity of each group was significantly decreased. There was no significant difference in the colony counts between the groups (P> 0.05). Conclusion PRG has a significant inhibitory effect on both MSSA and MRSA within a certain period of time. The antimicrobial effect of PRG is positively correlated with platelet concentration.