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目的：研究环磷酰胺的免疫抑制作用机制。方法：以大鼠胸腺为对象，采用细胞凋亡的原位特异性染色及ＤＮＡ凝胶电泳的方法，观察不同剂量、不同时间环磷酰胺处理后胸腺细胞的凋亡现象。结果：剂量为２０和７０ｍｇ／ｋｇ的环磷酰胺可引起广泛的胸腺细胞凋亡。该作用最早发生在环磷酰胺处理后的８ｈ，１２～２４ｈ最明显，４８ｈ消失。结论：环磷酰胺的免疫抑制作用可能是通过诱导免疫细胞凋亡而产生的。
Objective: To study the immunosuppressive mechanism of cyclophosphamide. Methods: The thymus of rats was used as the target. The apoptotic thymocytes were observed by in situ specific staining and DNA gel electrophoresis. The apoptosis of thymocytes was observed after different doses of cyclophosphamide. Results: Cyclophosphamide at doses of 20 and 70 mg / kg caused extensive thymocyte apoptosis. The role of the earliest occurred in cyclophosphamide 8h, 12 ~ 24h most obvious disappear 48h. Conclusion: The immunosuppressive effect of cyclophosphamide may be caused by the induction of immune cell apoptosis.