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本文介绍氮-磷灵敏检出器的GLC法,可同时测定血清中呋氟尿嘧啶(商品名“Tegafur”)和5-氟尿嘧啶(5-FU)的含量。方法:兔血清样品1ml,用0.5MNaH_2PO_4 0.1ml 调节pH 至6.3后,以乙酸乙酯8ml 提取,有机相室温减压干燥,残余物溶于内标乳清酸(30μg/ml)的甲醇液0.1ml 中,再加甲基化试剂重氮甲烷的乙醚液0.1
This article introduces the GLC method for the detection of nitrofuoresin, which can measure the content of 5-fluorouracil and 5-fluorouracil (Tefururon) in serum at the same time. METHODS: Rabbit serum sample (1ml) was prepared by adjusting the pH to 6.3 with 0.5M NaH2PO4 0.1ml and extracting with ethyl acetate (8ml). The organic phase was dried under reduced pressure at room temperature. The residue was dissolved in methanolic solution of endoseric acid (30μg / ml) ml, plus methylated reagent diazomethane ether solution 0.1