锰原子附着在植物光系统Ⅱ(PSⅡ)反应中心D_1和D_2蛋白上,构成锰蛋白复合物,在外周天线蛋白(47,43kD)、3种水溶性蛋白(33,23,17ku)、无机离子Ca~(2+)和Cl~-的辅助调节下,吸收光能,通过S_0→S_4态的循环,将水分解,形成分子氧.用氯化钠溶液清洗去除23,17ku水溶性蛋白后,水分解酶中锰原子极易被PD(Phenylenediamine,苯二胺)和HQ(Hydroquinone,氢醌)之类还原剂攻击,由Mn≥3+还原形成Mn~(2+)并释放出水分解酶,此过程伴随着水分解活性的相应降低. Manganese atoms are attached to the D_1 and D_2 proteins of PSⅡ reaction center to form manganese protein complexes, Under the auxiliary regulation of Ca ~ (2+) and Cl ~ -, the light energy is absorbed and the water is decomposed to form molecular oxygen through the circulation of S_0 → S_4 state. After washing with sodium chloride solution to remove 23,17ku of water-soluble protein, The manganese atom of hydrolase is easily attacked by reducing agents such as Phenylenediamine (PD) and hydroquinone (HQ), Mn (3+) is reduced to Mn 2+ to release the hydrolase, This process is accompanied by a corresponding decrease in water-splitting activity.