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目的:探讨特发性胎儿生长受限(IFGR)患者母血及脐血中胎盘生长因子(PLGF)及胎盘组织中Toll样受体2(TLR2)的表达及意义。方法:选取郑州大学第三附属医院2008年12月~2009年10月剖宫产分娩的FGR孕妇28例作为实验组,同期因社会因素剖宫产分娩的正常足月孕妇30例作为对照组。采用双抗体夹心酶联免疫吸附法(ELISA)测定实验组与对照组母血、脐血PLGF浓度;免疫组化SP法检测两组胎盘组织中TLR2的表达(平均灰度值为检测指标)。结果:①IFGR组母血、脐血PLGF浓度与对照组比较,明显降低(P<0.05);②胎盘组织中均有TLR2蛋白不同程度的阳性表达;③TLR2在胎盘合体滋养细胞上的表达实验组高于正常对照组,差异有统计学意义(P<0.05);④IFGR组母血中PLGF与胎盘组织中TLR2呈负相关。结论:母血、脐血PLGF减少可能是引起FGR的原因之一。IFGR组胎盘组织中TLR2表达增加可能是IFGR发病的重要因素之一。在IFGR中TLR2可能通过对PLGF的表达下调,抑制胎盘胎儿的发育。
Objective: To investigate the expression and significance of placental growth factor (PLGF) and Toll-like receptor 2 (TLR2) in maternal serum and cord blood of patients with idiopathic fetal growth restriction (IFGR). Methods: Twenty-eight FGR pregnant women delivered by cesarean section in the Third Affiliated Hospital of Zhengzhou University from December 2008 to October 2009 were selected as the experimental group. Thirty healthy full-term pregnant women delivered by cesarean section were selected as the control group. The concentration of PLGF in maternal blood and umbilical cord blood of experimental group and control group was measured by ELISA. The expression of TLR2 in placenta was detected by immunohistochemical SP method (the average gray value was the detection index). Results: (1) PLGF concentration in maternal blood and umbilical cord blood in IFGR group was significantly lower than that in control group (P <0.05); ② The expression of TLR2 protein in placenta was positive in some extent; ③ The expression of TLR2 in placental syncytiotrophoblast In the normal control group, the difference was statistically significant (P <0.05). (4) PLGF was negatively correlated with TLR2 in placenta. Conclusion: The decrease of PLGF in maternal and umbilical blood may be one of the causes of FGR. Increased expression of TLR2 in placenta of IFGR group may be one of the important factors in the pathogenesis of IFGR. In IFGR, TLR2 may inhibit the development of placenta fetus through the down-regulation of PLGF expression.